Journal: Scientific Reports

Article Title: Forensic significance of intracardiac expressions of Nrf2 in acute myocardial ischemia

doi: 10.1038/s41598-024-54530-x

Figure Lengend Snippet: Expression of Nrf2 in the human heart. ( A ) Immunohistochemical analysis using anti-Nrf2. Representative results from hearts of non-AIHD and AIHD groups are shown. Scale bar, 50μm. ( B ) Morphometrical analysis was performed to measure the number of Nrf2 + cells in each group. Mean and standard error of the number of Nrf2 + cells per high power field (hpf) in each group. ** P < 0.01 indicates a statistically significant difference. ( C ) Relationship between age, sex, or PMI and appearance of Nrf2 + cells in all cases; P values were determined using Spearman's correction coefficient by rank test as previously described .

Article Snippet: In this study, following polyclonal or monoclonal Abs (pAbs or mAbs) were used for immunohistochemical analyses in the present study: rabbit anti-human Nrf2 pAbs (bs-1074R, Bioss antibodies, Woburn, MA), rabbit anti-human fibronectin pAbs (15613-1-AP, Proteintec, Rosemont, IL), rabbit anti-human C5b-9 pAbs (bs-2673R, Bioss antibodies).

Techniques: Expressing, Immunohistochemical staining

Journal: Clinical and Translational Radiation Oncology

Article Title: Differential regulation of radioadaptation by quercetin between human normal and cancer cells

doi: 10.1016/j.ctro.2025.101099

Figure Lengend Snippet: Quercetin restores NRF2 nuclear translocation in radioadapted MCF10A cells. Representative immunofluorescence images of NRF2 (green) and DAPI (blue) and quantification of the nuclear-to-cytoplasmic NRF2 fluorescence ratio in MCF10A cells 24 h after 5 Gy irradiation with or without prior LDRT and quercetin treatment (Scale bar: 10 μm). Data is shown as mean ± SEM. * p < 0.05, ** p < 0.01.

Article Snippet: Cells were incubated with rabbit anti-human NRF2 primary antibody (1:200; Proteintech, 16396–1-AP), and detection was performed using goat anti-rabbit Alexa Fluor 488 (1:500, Invitrogen, 11001).

Techniques: Translocation Assay, Immunofluorescence, Fluorescence, Irradiation

Journal: Molecules

Article Title: Dimethyl Bisphenolate Ameliorates Carbon Tetrachloride-Induced Liver Injury by Regulating Oxidative Stress-Related Genes

doi: 10.3390/molecules28247989

Figure Lengend Snippet: DMB ameliorated the Nrf2-related factors in CCl 4 -induced liver injury. ( A ) The related protein Nrf2 of DMB was predicted; ( B ) DMB increased the content of GSH in the liver tissues of CCl 4 -treated rats. Data were abnormally distributed, and they are expressed as median (interquartile range), * p < 0.05, ** p < 0.01; Kruskal–Wallis H test. ( C ) DMB reduced the MDA levels in the liver tissues of CCl 4 -treated rats. Data are expressed as median (interquartile range), * p < 0.05, ** p < 0.01; Kruskal–Wallis H test. ( D ) Western blot analysis of Nrf2-related gene expression in the liver tissues; ( E ) Relative GCLC expression in the liver tissues. Data are presented as the mean ± SD. * p < 0.05; ** p < 0.01; ANOVA. ( F ) Relative Nrf2 expression in the liver tissues. Data are presented as the mean ± SD. * p < 0.05; ** p < 0.01; ANOVA. ( G ) Relative p53 expression in the liver tissues. Data are presented as the mean ± SD. ** p < 0.01; ANOVA. ( H ) DMB decreased the expression of Bax but increased Bcl2 expression in the liver tissues. Data are presented as the mean ± SD. * p < 0.05; ** p < 0.01; NS, no statistical difference.

Article Snippet: Cell slides were washed with PBS and fixed at room temperature with 4% paraformaldehyde (BL539A, Biosharp, China) for 2 h. They were then broken using 0.2% TritonX-100 at room temperature for 15 min and sealed with a sealing solution at room temperature for 1 h. The first antibody rabbit anti-human Nrf2 (1:100, Bioworld Technology, Ltd., MN, USA) was incubated overnight, and the second antibody was incubated at 37 °C in the dark for 2 h and then incubated with DAPI (C1006, Beyotime Biotechnology, Shanghai, China) for 5 min.

Techniques: Western Blot, Expressing

Journal: Molecules

Article Title: Dimethyl Bisphenolate Ameliorates Carbon Tetrachloride-Induced Liver Injury by Regulating Oxidative Stress-Related Genes

doi: 10.3390/molecules28247989

Figure Lengend Snippet: DMB reversed CCl 4 -induced Nrf2 and GCLC expression. ( A , B ) Western blot detected the expression of Nrf2 and GCLC in HepG2 and MHCC-97H cells, respectively. Data are presented as the mean ± SD. ** p < 0.01; ANOVA. ( C , D ) Immunofluorescence analyses of the expression of Nrf2 in HepG2 and MHCC-97H cells, respectively. Bar = 50 μm.

Article Snippet: Cell slides were washed with PBS and fixed at room temperature with 4% paraformaldehyde (BL539A, Biosharp, China) for 2 h. They were then broken using 0.2% TritonX-100 at room temperature for 15 min and sealed with a sealing solution at room temperature for 1 h. The first antibody rabbit anti-human Nrf2 (1:100, Bioworld Technology, Ltd., MN, USA) was incubated overnight, and the second antibody was incubated at 37 °C in the dark for 2 h and then incubated with DAPI (C1006, Beyotime Biotechnology, Shanghai, China) for 5 min.

Techniques: Expressing, Western Blot, Immunofluorescence